Figure 1 Shows the GAB-KGB DNA lacking documentation and figure 1 A displays the Hell cells with just GAP DNA The Hell cells with GAP DNA and documentation (Figure 1 B) fluoresced throughout the entire cell including the nucleus most likely caused by the small size of the fluorescent protein used. However, when GAP-KGB DNA that lacked the documentation was analyzed, the entire cell fluoresced similarly except for the nucleus (Figure 1 C), indicating that the protein does not contain a nuclear localization signal. The nucleus is fluoresced at a high intensity when documentation was added to the
GAP-KGB DNA containing cells (Figure I D) because the protein was capable of passing through to the nucleus. When the glorification hormone documentation binds to KGB, a conformational change causes neighboring proteins to dissociate and reveal the nuclear localization signal, therefore fluorescing the nucleus These results conclude that the GIBE-KGB DNA was successfully transferred into the Hell cells and through the addition of documentation, the glorification receptor protein with an activated glorification-binding domain localized to the nucleus following a conformational change.